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vectastain abc amp rabbit mouse igg kit  (Vector Laboratories)


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    Vector Laboratories vectastain abc amp rabbit mouse igg kit
    Vectastain Abc Amp Rabbit Mouse Igg Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 531 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+igg+vectastain+abc+amp+kit/Unconjugated+Rabbit+Anti-Rat+IgG+Antibody%2C+mouse+adsorbed/pmc04352142-105-26-30
    Average 94 stars, based on 531 article reviews
    vectastain abc amp rabbit mouse igg kit - by Bioz Stars, 2026-10
    94/100 stars

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    Membrane:

    Article Title: Circadian rhythms of gastrointestinal function are regulated by both central and peripheral oscillators
    Article Snippet: The membrane was rinsed in PBS for 5 min and then blocked in 5% nonfat dry milk in PBS with 0.1% Tween 20 (PBT) for 30 min. Rabbit polyclonal IgG anti-TH antibody (EMD Millipore) was added at a 1:1,000 dilution and left overnight on a rotator at 4°C. .. The membrane was blocked for 7 min in 5% nonfat dry milk in PBT twice and washed in 1× casein (rabbit IgG Vectastain ABC-Amp Kit, Vector Laboratories) for 7 min. .. The membrane was incubated in biotinylated anti-rabbit IgG (rabbit IgG Vectastain ABC-Amp Kit) for 40 min. After two 7-min washes in PBT with 5% milk and one 7-min wash in 1× casein solution, the membrane was incubated for 30 min in ABC-Amp solution (rabbit IgG Vectastain ABC-Amp Kit) and then washed three times in 1× casein for 7 min. For equilibration, the membrane was incubated in 0.1 M Tris buffer (pH 9.5) for 10 min. Immunoreactive bands were detected using an alkaline phosphatase substrate staining solution [Alkaline Phosphatase Substrate Kit IV 5-bromo-4-chloro-3′-indoyl phosphate (BCIP/NBT), Vector Laboratories].



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